APO-Easy®: Genotyping kit for the determination of two APOE SNPs related to AD.
The APOE gene on chromosome 19, responsible for coding apolipo-protein E, is genetically linked to both common late-onset familial and sporadic forms of AD. The human APOE gene comprises three alleles: -ε2, -ε3, and -ε4 distributed in the population at 7%, 79%, and 14%, respectively.
Having one or two copies of the ε4 allele in APOE gene is statistically associated with an increased risk of developing AD. Approximately 65% of AD patients in the Caucasian population carry the APOE ε4 allele.
Backed by clinical science, the APO-Easy® Genotyping kit is a result of an extensive research, an innovative and highly-sensitive product clinicially validated by multicentric clinical studies in 7 countries on ~960 patients with neurodegenerative diseases including patients with Mild Cognitive Impairment (MCI), mild and moderate to severe AD, Frontotemporal Dementia (FTD), Lewy Body Dementia (LBD), Parkinson Disease Dementia (PDD), Cortico-Basal Degeneration (CBD) and Progressive Supranuclear Palsy (PSP).
The APO-Easy® Genotyping kit is a qualitative in vitro diagnostic (IVD) test based on qPCR intended for the detection of two single-nucleotide-polymorphisms (SNP) rs429358 and rs7412 in human genomic DNA from whole blood, saliva and buccal swab through the use of fluorescently labeled probes (FAM and VIC) allowing allelic discrimination and determination of APOE genotypes.
- 100% specific, reliable and accurate
- No false positive or false negative results
- Determination of -ε2, -ε3, and -ε4 alleles
accuracy rate
reduction in master mix volume
hours
limit of detection
Streamlined process
extract
run
analyze
qPCR genotyping
AD genotyping involves identifying genetic variations, using molecular biology techniques such as PCR, Sanger sequencing, and Next-Generation Sequencing (NGS). Method selection depends on study goals, scale, cost, and desired resolution.
Sanger sequencing : Moderate
NGS : High
qPCR genotyping : Low to moderate
Sanger sequencing : Days
NGS : Days to weeks
qPCR genotyping : Hours
Sanger sequencing : Low
NGS : High
qPCR genotyping : Moderate to high
Key assay parameters
Kit storage
Operating time
30 minutes for plate preparation and 1 hour and 29 minutes for the qPCR cycle.
Sample storage
Assay validation
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through biomarker discovery, development and regulatory qualification.